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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
12/12/2012 |
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Data da última atualização: |
09/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NASCIMENTO, C. S.; PEIXOTO, J. de O.; VERARDO, L. L.; CAMPOS, C. F.; WELLER, M. M. C.; FARIA, V. R.; BOTELHO, M. E.; MARTINS, M. F.; MACHADO, M. A.; SILVA, F. F.; LOPES, P. S.; GUIMARÃES, S. E. F. |
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Afiliação: |
C. S. NASCIMENTO, UFV; JANE DE OLIVEIRA PEIXOTO, CNPSA; L. L. VERARDO, UFV; C. F. CAMPOS, UFV; M. M. C. WELLER, UFV; V. R. FARIA, UFV; M. E. BOTELHO, UFV; MARTA FONSECA MARTINS, CNPGL; MARCO ANTONIO MACHADO, CNPGL; F. F. SILVA, UFV; P. S. LOPES, UFV; S. E. F. GUIMARÃES, UFV. |
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Título: |
Transcript profiling of expressed sequence tag from semimembranosus muscle of commercial and naturalized pig breeds. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 11, n. 3, p. 3315-3328. 2012. |
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DOI: |
https://doi.org/10.4238/2012.june.15.1 |
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Idioma: |
Inglês |
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Conteúdo: |
In general, genetic differences across different breeds of pig lead to variation in mature body size and slaughter age. The Commercial breeds Duroc and Large White and the local Brazilian breed Piau are ostensibly distinct in terms of growth and muscularity, commercial breeds are much leaner while local breeds grow much slower and are fat type pigs. However, the genetic factors that underlie such distinctions remain unclear. We used expressed sequence tags (ESTs) to characterize and compare transcript profiles in the semimembranosus muscle of these pig breeds. Our aim was to identify differences in breed-related gene expression that might influence growth performance and meat quality. We constructed three non-normalized cDNA libraries from semimembranosus muscle, using two samples from each one, of these three breeds; 6902 high-quality ESTs were obtained. Cluster analysis was performed and these sequences were clustered into 3670 unique sequences; 24.7% of the sequences were categorized as contigs and 75.3% of the sequences were singletons. Based on homology searches against the SwissProt protein database, we were able to assign a putative protein identity to only 1050 unique sequences. Among these, 58.5% were full-length protein sequences and 17.2% were pig-specific sequences. Muscle structural and cytoskeletal proteins, such as actin, and myosin, were the most abundant transcripts (16.7%) followed by those related to mitochondrial function (12.9%), and ribosomal proteins (12.4%). Furthermore, ESTs generated in this study provide a rich source for identification of novel genes and for the comparative analysis of gene expression patterns in divergent pig breeds. MenosIn general, genetic differences across different breeds of pig lead to variation in mature body size and slaughter age. The Commercial breeds Duroc and Large White and the local Brazilian breed Piau are ostensibly distinct in terms of growth and muscularity, commercial breeds are much leaner while local breeds grow much slower and are fat type pigs. However, the genetic factors that underlie such distinctions remain unclear. We used expressed sequence tags (ESTs) to characterize and compare transcript profiles in the semimembranosus muscle of these pig breeds. Our aim was to identify differences in breed-related gene expression that might influence growth performance and meat quality. We constructed three non-normalized cDNA libraries from semimembranosus muscle, using two samples from each one, of these three breeds; 6902 high-quality ESTs were obtained. Cluster analysis was performed and these sequences were clustered into 3670 unique sequences; 24.7% of the sequences were categorized as contigs and 75.3% of the sequences were singletons. Based on homology searches against the SwissProt protein database, we were able to assign a putative protein identity to only 1050 unique sequences. Among these, 58.5% were full-length protein sequences and 17.2% were pig-specific sequences. Muscle structural and cytoskeletal proteins, such as actin, and myosin, were the most abundant transcripts (16.7%) followed by those related to mitochondrial function (12.9%), and ribosomal proteins (... Mostrar Tudo |
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Palavras-Chave: |
CDNA; Etiquetas de sequencia expressa. |
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Thesagro: |
Músculo; Presunto. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/942276/1/Transcript-profiling-of-expressed-sequence-tag-from-semimembranosus-muscle.pdf
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Marc: |
LEADER 02646naa a2200313 a 4500
001 1942276
005 2024-02-09
008 2012 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4238/2012.june.15.1$2DOI
100 1 $aNASCIMENTO, C. S.
245 $aTranscript profiling of expressed sequence tag from semimembranosus muscle of commercial and naturalized pig breeds.$h[electronic resource]
260 $c2012
520 $aIn general, genetic differences across different breeds of pig lead to variation in mature body size and slaughter age. The Commercial breeds Duroc and Large White and the local Brazilian breed Piau are ostensibly distinct in terms of growth and muscularity, commercial breeds are much leaner while local breeds grow much slower and are fat type pigs. However, the genetic factors that underlie such distinctions remain unclear. We used expressed sequence tags (ESTs) to characterize and compare transcript profiles in the semimembranosus muscle of these pig breeds. Our aim was to identify differences in breed-related gene expression that might influence growth performance and meat quality. We constructed three non-normalized cDNA libraries from semimembranosus muscle, using two samples from each one, of these three breeds; 6902 high-quality ESTs were obtained. Cluster analysis was performed and these sequences were clustered into 3670 unique sequences; 24.7% of the sequences were categorized as contigs and 75.3% of the sequences were singletons. Based on homology searches against the SwissProt protein database, we were able to assign a putative protein identity to only 1050 unique sequences. Among these, 58.5% were full-length protein sequences and 17.2% were pig-specific sequences. Muscle structural and cytoskeletal proteins, such as actin, and myosin, were the most abundant transcripts (16.7%) followed by those related to mitochondrial function (12.9%), and ribosomal proteins (12.4%). Furthermore, ESTs generated in this study provide a rich source for identification of novel genes and for the comparative analysis of gene expression patterns in divergent pig breeds.
650 $aMúsculo
650 $aPresunto
653 $aCDNA
653 $aEtiquetas de sequencia expressa
700 1 $aPEIXOTO, J. de O.
700 1 $aVERARDO, L. L.
700 1 $aCAMPOS, C. F.
700 1 $aWELLER, M. M. C.
700 1 $aFARIA, V. R.
700 1 $aBOTELHO, M. E.
700 1 $aMARTINS, M. F.
700 1 $aMACHADO, M. A.
700 1 $aSILVA, F. F.
700 1 $aLOPES, P. S.
700 1 $aGUIMARÃES, S. E. F.
773 $tGenetics and Molecular Research$gv. 11, n. 3, p. 3315-3328. 2012.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 27 | |
| 2. |  | SILVA, M. V. G. B.; VERARDO, L. L.; MACHADO, M. A.; PANETTO, J. C. do C.; CAROLINO, I.; CAROLINO, N. Candidate genes for disease, reproduction and meat quality traits in Portuguese native breeds. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 12., 2022, Rotterdam. Proceedings... Rotterdam: [s.n.], 2022.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
|    |
| 3. | | VERARDO, L. L.; SILVA, F. F.; VARONA, L.; RESENDE, M. D. V. de; BASTIAANSEN, J. W. M.; LOPES, P. S.; GUIMARÃES, S. E. F. Bayesian GWAS and network analysis revealed new candidate genes for number of teats in pigs. Journal of Applied Genetics, v. 56, n. 1, p. 123-132, Feb. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Florestas. |
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| 4. | | CARVALHO, L. S.; ALMEIDA, E. A. R.; SOUZA, K. G. L. de; SILVA, M. V. G. B.; MAGALHÃES, A. F. B.; VERARDO, L. L. Análise funcional de genes candidatos associados à resistência ao carrapato em bovinos da raça Gir. In: SIMPÓSIO DE PRODUÇÃO ANIMAL, 7.; SIMPÓSIO DO PROGRAMA DE PÓS-GRADUAÇÃO, 2.; SEMANA DA ZOOTECNIA, 15., 2023, Diamantina. Rumo ao futuro: [anais]. Diamantina: Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2023. p. 24-26.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 5. | | ALMEIDA, E. A. R.; PEREIRA, J. R.; CARVALHO, L. S.; SILVA, M. V. G. B.; MAGALHÃES, A. F. B.; VERARDO, L. L. Análises pós-estudo de associação no genoma de bovinos da raça Gir para a identificação de genes candidatos para perfil de ácidos graxos no leite. In: SIMPÓSIO DE PRODUÇÃO ANIMAL, 7.; SIMPÓSIO DO PROGRAMA DE PÓS-GRADUAÇÃO, 2.; SEMANA DA ZOOTECNIA, 15., 2023, Diamantina. Rumo ao futuro: [anais]. Diamantina: Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2023. p. 36-38.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | FERNANDES, A. A. S.; VIEIRA, J. I. G.; FERREIRA, P. H.; MAGALHÃES, A. F. B.; SILVA, M. V. G. B.; VERARDO, L. L. An insertion in Caracu breed genome with a possible role in fatty acids profile. In: REUNIÃO DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 57., 2022, Campinas. Tropical animal science and pratice to feed the planet: proceedings. Brasília, DF: Sociedade Brasileira de Zootecnia; São Carlos, SP: Embrapa Pecuária Sudeste, 2022. p. 232.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | PANETTO, J. C. do C.; VERARDO, L. L.; MACHADO, M. A.; MARTINS, M. F.; FAZA, D. R. L. R.; SILVA, M. V. G. B. Genotype by environment interaction in Brazilian Dairy Gir cattle. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 11., 2018, Auckland. Proceedings... [S.l.: s.n.], 2018.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | CAMPOS, B. M.; CARMO, A. S. do; SILVA, T. B. R. da; VERARDO, L. L.; GOUVEIA, J. J. de S.; MALHADO, C. H. M.; SILVA, M. V. G. B.; CARNEIRO, P. L. S. Identification of artificial selection signatures in Caracu breed lines selected for milk production and meat production. Livestock Science, v. 206, p. 82-87, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | VERARDO, L. L.; NASCIMENTO, C. S.; SILVA, F. F.; GASPARINO, E.; MARTINS, M. F.; TORIYAMA, E.; FARIA, V. R.; BOTELHO, M. E.; COSTA, K. A.; LOPES, P. S.; GUIMARÃES, S. E. F. Identification and validation of differentially expressed genes from pig skeletal muscle. Journal of Animal Breeding and Genetics, v. 130, n. 5, p. 372-381, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | VERARDO, L. L.; OTTO, P. I.; MACHADO, M. A.; PANETTO, J. C. do C.; REIS, D. R. de L.; EGITO, A. A. do; VITORINO, A. S. M.; CAROLINO, M. I. C. M.; CAROLINO, N. P.; SILVA, M. V. G. B. Exploring the genetic origin of Brazilian locally adapted breeds: admixture, population history and relationship with Portuguese and indicine cattle. Livestock Science, v. 282, 105455, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | DUARTE, D. A. S.; FORTES, M. R. S.; DUARTE, M. de S.; GUIMARÃES, S. E. F.; VERARDO, L. L.; VERONEZE, R.; RIBEIRO, A. M. F.; LOPES, P. S.; RESENDE, M. D. V. de; SILVA, F. F. e. Genome-wide association studies, meta-analyses and derived gene network for meat quality and carcass traits in pigs. Animal Production Science, v. 58, n. 6, p. 1100-1008, May 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Florestas. |
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| 12. | | VERARDO, L. L.; STAFUZZA, N. B.; MUNARI, D. P.; ZERLOTINI NETO, A.; CHUD, T. C. S.; GARRICK, D. J.; COLE, J. B.; PANETTO, J. C. do C.; MACHADO, M. A.; MARTINS, M. F.; SILVA, M. V. G. B. A gene-transcription factor network associated with residual feed intake based on SNVs/InDels identified in Gir, Girolando and Holstein cattle breeds. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 11., 2018, Auckland. Proceedings... [S.l.: s.n.], 2018.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | VERARDO, L. L.; STAFUZZA, N. B.; MUNARI, D. P.; ZERLOTINI NETO, A.; CHUD, T. C. S.; GARRICK, D. J.; COLE, J. B.; PANETTO, J. C. do C.; MACHADO, M. A.; MARTINS, M. F.; SILVA, M. V. G. B. A gene-transcription factor network associated with residual feed intake based on SNVs/InDels identified in Gir, Girolando and Holstein cattle breeds. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 11., 2018, Auckland. Proceedings... [S.l.: s.n.], 2018. 6 p. Na publicação: A. Zerlotini, J. C. C. Panetto. WCGALP 2018.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 14. | | PEREIRA, H. P.; VERARDO, L. L.; WELLER, M. M. D. C. A.; SBARDELLA, A. P.; MUNARI, D. P.; DAIBERT, R. M. de P.; CARVALHO, W. A.; MACHADO, M. A.; MARTINS, M. F. Going further post-RNA-seq: in silico functional analyses revealing candidate genes and regulatory elements related to mastitis in dairy cattle. Journal of Dairy Research, v. 88, p. 286-292, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | NASCIMENTO, C. S.; PEIXOTO, J. de O.; VERARDO, L. L.; CAMPOS, C. F.; WELLER, M. M. C.; FARIA, V. R.; BOTELHO, M. E.; MARTINS, M. F.; MACHADO, M. A.; SILVA, F. F.; LOPES, P. S.; GUIMARÃES, S. E. F. Transcript profiling of expressed sequence tag from semimembranosus muscle of commercial and naturalized pig breeds. Genetics and Molecular Research, v. 11, n. 3, p. 3315-3328. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | VIEIRA, J. I. G.; BRAGA, L. G.; CHU, T. C. S.; FERREIRA, P. H.; GUIMARÃES, S. E. F.; MARTINS, M. F.; PANETTO, J. C. do C.; MACHADO, M. A.; SILVA, D. B. dos S.; BONAFÉ, C. M.; MAGALHÃES, A. F. B.; SILVA, M. V. G. B.; VERARDO, L. L. Resequencing of Brazilian locally adapted cattle breeds revealed variants in candidate genes and transcription factors for meat fatty acid profile. Journal of Animal Breeding and Genetics, 2024. First online.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | SILVA, M. V. G. B.; MARTINS, M. F.; CEMBRANELLI, M. de A. R.; PAIVA, L. DE C.; PANETTO, J. C. do C.; MACHADO, M. A.; VERARDO, L. L.; GONÇALVES, G. S.; REIS, D. R. de L.; MENDONÇA JÚNIOR, C. F.; BRAGA, F. do A.; FRANZON, C. de A. Programa de melhoramento genético da raça Girolando - Avaliação genômica de fêmeas jovens - junho/2017. Juiz de Fora: Embrapa Gado de Leite, 2017. 20 p. (Embrapa Gado de Leite. Documentos, 205)| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | PAIXÃO, D. M.; CARNEIRO, P. L. S.; PAIVA, S. R.; SOUSA, K. R. S.; VERARDO, L. L.; BRACCINI NETO, J.; PINTO, A. P. G.; HIDALGO, A. M.; NASCIMENTO, C. S. do; PÉRISSÉ, I. V.; LOPES, P. S.; GUIMARÃES, S. E. F. Mapeamento de QTL nos cromossomos 1, 2, 3, 12, 14, 15 e X em suínos: características de carcaça e qualidade de carne. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 64, n. 4, p. 974-982, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | TEIXEIRA, F. R. F.; NASCIMENTO, M.; NASCIMENTO, A. C. C.; SILVA, F. F. e; CRUZ, C. D.; AZEVEDO, C. F.; PAIXÃO, D. M.; BARROSO, L. M. A.; VERARDO, L. L.; RESENDE, M. D. V. de; GUIMARÃES, S. E. F.; LOPES, P. S. Factor analysis applied to genome prediction for high-dimensional phenotypes in pigs. Genetics and Molecular Research, v. 15, n. 2, 2016. 10 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Florestas. |
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| 20. | | CAMPOS, B. M.; CARMO, A. S. do; EGITO, A. A. do; MARIANTE, A. da S.; ALBUQUERQUE, M. S. M. do; GOUVEIA, J. J. S. de; MALHADO, C. H. M.; VERARDO, L. L.; SILVA, M. V. G. B. da; CARNEIRO, P. L. S. Genetic diversity, population structure, and correlations between locally adapted zebu and taurine breeds in Brazil using SNP markers. Tropical Animal Health and Production, v. 49, n. 8, p 1677-1684, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 27 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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